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Content Determination of Hesperidin in Xiaoermoji Tablets by HPLC

ER Fengjun

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Abstract

OBJECTIVE:To establish HPLC method for the determination of hesperidin in xiaoermoji tablets.METHODS:The determination was performed on Spherisorb C 18 ,the mobile phase consisted of methanol-glacial acetic acid-water(25∶4∶71),the detection wavelength was283nm,the flow rate was2.0ml/min,the column temperature was50℃,the sensitivity was0.16AUFS and the sample size was20μl.RESULTS:Hesperidin has a good linear relation with peak area score when its sample size was within a range of0.024μg～1.2μg(r=0.9999),the average recovery was99.1%(RSD=0.8%).CONCLUSION:The method is simple,fast,sensitive and accurate,and it can be served as a basis for the quality control of xiaoermoji tablets.

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What this paper is about

OBJECTIVE:To establish HPLC method for the determination of hesperidin in xiaoermoji tablets.METHODS:The determination was performed on Spherisorb C 18 ,the mobile phase consisted of methanol-glacial acetic acid-water(25∶4∶71),the detection wavelength was283nm,the flow rate was2.0ml/min,the column temperature was50℃,the sensitivity was0.16AUFS and the sample size was20μl.RESULTS:Hesperidin has a good linear relation with peak area score when its sample size was within a range of0.024μg～1.2μg(r=0.9999),the average recovery was99.1%(RSD=0.8%).CONCLUSION:The method is simple,fast,sensitive and accurate,and it can be served as a basis for the quality control of xiaoermoji tablets.

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Available abstract

OBJECTIVE:To establish HPLC method for the determination of hesperidin in xiaoermoji tablets.METHODS:The determination was performed on Spherisorb C 18 ,the mobile phase consisted of methanol-glacial acetic acid-water(25∶4∶71),the detection wavelength was283nm,the flow rate was2.0ml/min,the column temperature was50℃,the sensitivity was0.16AUFS and the sample size was20μl.RESULTS:Hesperidin has a good linear relation with peak area score when its sample size was within a range of0.024μg～1.2μg(r=0.9999),the average recovery was99.1%(RSD=0.8%).CONCLUSION:The method is simple,fast,sensitive and accurate,and it can be served as a basis for the quality control of xiaoermoji tablets.

Key concepts: Hesperidin, Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Linear relationship, Linear range, Mathematics

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Content Determination of Hesperidin in Xiaoermoji Tablets by HPLC — Research Paper | ScholarLens