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2008•The Chinese Journal of Modern Applied PharmacyRequires access

Determination of Content of Hesperidin in Weixuekang Tablet by HPLC

YU De-cai

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Abstract

OBJECTIVE To establish a HPLC method for determination of hesperidin in Weixuekang tablets.METHODS The C18 column was used with CH3CN-0.1%H3PO4(20∶80)as the mobile phase.The flow rate was 1.0 mL·min-1 and the detection wave length was 283 nm.The column temperature was 35 ℃,and the sample size was 10 μL.RESULTS The calibration curve of hesperidin was linear within the range of 0.059 2-0.296 0 μg(r=0.999 9).The average recovery rate was 98.13%(RSD=1.54%).CONCLUSION The established method is convenient,accurate,as well as reproducible,and is applicable for the quality control of hesperidin in Weixuekang tablets.

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What this paper is about

OBJECTIVE To establish a HPLC method for determination of hesperidin in Weixuekang tablets.METHODS The C18 column was used with CH3CN-0.1%H3PO4(20∶80)as the mobile phase.The flow rate was 1.0 mL·min-1 and the detection wave length was 283 nm.The column temperature was 35 ℃,and the sample size was 10 μL.RESULTS The calibration curve of hesperidin was linear within the range of 0.059 2-0.296 0 μg(r=0.999 9).The average recovery rate was 98.13%(RSD=1.54%).CONCLUSION The established method is convenient,accurate,as well as reproducible,and is applicable for the quality control of hesperidin in Weixuekang tablets.

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Available abstract

OBJECTIVE To establish a HPLC method for determination of hesperidin in Weixuekang tablets.METHODS The C18 column was used with CH3CN-0.1%H3PO4(20∶80)as the mobile phase.The flow rate was 1.0 mL·min-1 and the detection wave length was 283 nm.The column temperature was 35 ℃,and the sample size was 10 μL.RESULTS The calibration curve of hesperidin was linear within the range of 0.059 2-0.296 0 μg(r=0.999 9).The average recovery rate was 98.13%(RSD=1.54%).CONCLUSION The established method is convenient,accurate,as well as reproducible,and is applicable for the quality control of hesperidin in Weixuekang tablets.

Key concepts: Hesperidin, Chromatography, Calibration curve, High-performance liquid chromatography, Content determination, Chemistry, Linear relationship, Recovery rate

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