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2011•Zhongguo shiyan fangjixue zazhiRequires access

Determination Content of Hesperidin in Xinjianwei Tablets by HPLC

Xiaofeng Zhang

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Abstract

Objective:To establish a HPLC method for the determination of hesperidin in Xinjianwei tablets.Method: Phenomenex C18(4.6 mm × 250 mm,5 μm) column was used.The mobile phase consisted of methanol-acetic acid-water(35∶ 4∶ 61) and the flow rate was 1.0 mL.min-1,the detective wavelength was at 283 nm.Result: The hesperidin has a good linear in the range of 0.10-2.03 μg(r = 0.999 9),the average recovery(n = 9) was 98.37%.Conclusion:The method is rapid,sensitive,and accurate.It can be used for the quality control of Xinjianwei tablets.

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What this paper is about

Objective:To establish a HPLC method for the determination of hesperidin in Xinjianwei tablets.Method: Phenomenex C18(4.6 mm × 250 mm,5 μm) column was used.The mobile phase consisted of methanol-acetic acid-water(35∶ 4∶ 61) and the flow rate was 1.0 mL.min-1,the detective wavelength was at 283 nm.Result: The hesperidin has a good linear in the range of 0.10-2.03 μg(r = 0.999 9),the average recovery(n = 9) was 98.37%.Conclusion:The method is rapid,sensitive,and accurate.It can be used for the quality control of Xinjianwei tablets.

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Available abstract

Objective:To establish a HPLC method for the determination of hesperidin in Xinjianwei tablets.Method: Phenomenex C18(4.6 mm × 250 mm,5 μm) column was used.The mobile phase consisted of methanol-acetic acid-water(35∶ 4∶ 61) and the flow rate was 1.0 mL.min-1,the detective wavelength was at 283 nm.Result: The hesperidin has a good linear in the range of 0.10-2.03 μg(r = 0.999 9),the average recovery(n = 9) was 98.37%.Conclusion:The method is rapid,sensitive,and accurate.It can be used for the quality control of Xinjianwei tablets.

Key concepts: Hesperidin, Chromatography, High-performance liquid chromatography, Content determination, Chemistry, Acetic acid, Linear range, Detection limit

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