Apoptosis of human hepatocellular carcinoma cell line HepG2 induced by anti-human DR5 monoclonal antibody

To evaluate the effect of anti-human DR5 monoclonal antibody(mDRA-6) on the apoptosis of human hepatocellular carcinoma cell line HepG2,the surface expression of DR5 molecule on HepG2 cells was detected by flow cytometry,and the cytotoxicity induced by mDRA-6 on HepG2 cells was assayed with MTT methodThe rate of apoptosis was determined by flow cytometry with Annexin V-FITC/PI staining,and the influence of mDRA-6 on the morphology of HepG2 cells was observed under fluorescent microscopy.In the experimental results,it was found that the DR5 molecules could be detected on the surface of HepG2 cells with a positive detection rate of 40%.As demonstrated by the MTT assay,the inhibitory rate on the proliferation of HepG2 cells accounted up to be 42% under treatment of 40 mg/L of mDRA6.The rate of apoptosis of cells induced by 3 mg/L of mDRA-6 was 24.61% in the presence of 3 mg/L of mDRA-6 for 6 hours,as demostrated by the analysis with flow cytometry assay.with Annexin V-FITC/PI staining.It is evident from the above observations that the anti-human DR5 monoclonal antibody can induce apoptosis of HepG2 cells.in vitro.