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Study on HPLC-specific chromatograms of Flos Lonicerae Japonicae

Cheng Ming-chuan

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Abstract

Objective:To establish the HPLC specific chromatograms of Flos Lonicerae Japonicae,it may provide the basis for identification for quality of Flos Lonicerae Japonicae.Methods:Chromatographic analysis was achieved on a Phnomenex Gemini C_(18) column(250 mm×4.6 mm,5 μm) using a mobile phase of 0.5% phosphoric acid- acetonitrile eluted with a linear gradient.The detectors were consisted of UV and ECD detector.The flow rate was maintained at 1.0 mL·min~(-1),with the column temperature at 40 ℃.Results:The method provided two kinds of specific chromatogram with excellent separation,which were HPLC-UV (238 nm) and HPLC-ECD (250 mV) specific chromatograms.They had a good correlation and complementation with each other.Seventeen common peaks and nine common peaks were confirmed in each specific chromatogram,respectively.Conclusion:The method is sta- ble,accurate and creditable.It can be used for the quality control of Flos Lonicerae Japonicae.

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What this paper is about

Objective:To establish the HPLC specific chromatograms of Flos Lonicerae Japonicae,it may provide the basis for identification for quality of Flos Lonicerae Japonicae.Methods:Chromatographic analysis was achieved on a Phnomenex Gemini C_(18) column(250 mm×4.6 mm,5 μm) using a mobile phase of 0.5% phosphoric acid- acetonitrile eluted with a linear gradient.The detectors were consisted of UV and ECD detector.The flow rate was maintained at 1.0 mL·min~(-1),with the column temperature at 40 ℃.Results:The method provided two kinds of specific chromatogram with excellent separation,which were HPLC-UV (238 nm) and HPLC-ECD (250 mV) specific chromatograms.They had a good correlation and complementation with each other.Seventeen common peaks and nine common peaks were confirmed in each specific chromatogram,respectively.Conclusion:The method is sta- ble,accurate and creditable.It can be used for the quality control of Flos Lonicerae Japonicae.

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Available abstract

Objective:To establish the HPLC specific chromatograms of Flos Lonicerae Japonicae,it may provide the basis for identification for quality of Flos Lonicerae Japonicae.Methods:Chromatographic analysis was achieved on a Phnomenex Gemini C_(18) column(250 mm×4.6 mm,5 μm) using a mobile phase of 0.5% phosphoric acid- acetonitrile eluted with a linear gradient.The detectors were consisted of UV and ECD detector.The flow rate was maintained at 1.0 mL·min~(-1),with the column temperature at 40 ℃.Results:The method provided two kinds of specific chromatogram with excellent separation,which were HPLC-UV (238 nm) and HPLC-ECD (250 mV) specific chromatograms.They had a good correlation and complementation with each other.Seventeen common peaks and nine common peaks were confirmed in each specific chromatogram,respectively.Conclusion:The method is sta- ble,accurate and creditable.It can be used for the quality control of Flos Lonicerae Japonicae.

Key concepts: Flos, Chemistry, Chromatography, High-performance liquid chromatography, Gradient elution, Phosphoric acid, Elution, Rutin

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