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[Determination of loniceroside A and C in Lonicerae japonicae by RP-HPLC].

Xiaoguang Zhang, Zhimin Wang, Xiaomei Song, Jingjing Zhu, Huimin Gao, Limei Lin

Abstract

OBJECTIVE: To develop an HPLC method for determination of loniceroside A and loniceroside C in Lonicerae japonicae. METHOD: The analysis was carried out on an Alltech C18 column (4.6 mm x 250 mm, 5 microm) evaluated with methanol-acetonitrile-0.1% glacial acetic acid as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 210 nm for UV detection. RESULT: The calibration curves were linear over the range from 0.15 to 2.25 microg (r = 0.999 9) for loniceroside A and 0.11 to 1.65 microg (r = 0.999 1) for loniceroside C. The average recoveries were 99.9% and 98.3%, respectively. CONCLUSION: The contents of Loniceroside A&C are diverse in Flos Lonicerae japonicae in different regions.

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Key concepts: Chromatography, High-performance liquid chromatography, Calibration curve, Chemistry, Acetic acid, Analytical Chemistry (journal), Detection limit, Biochemistry