ScholarLens Back to search
2009•Yaowu fenxi zazhiRequires access

Optimization of an HPLC method for determination of luteolin-7-O-glucoside in Flos Lonicerae Japonicae

Lin Rui-chao

Open publisher page 2 citations

Abstract

Objective:To develop an HPLC method for the determination of luteolin-7-O-glucoside in Flos Lonicerae Japonicae.Method:An Agilent ZORBAX SB-phenyl column(4.6 mm×250 mm,5 μm) was adopted;The mobile phase was acetonitrile-0.5% acetic acid with gradient elution mode at a flow rate of 1 mL·min-1;The detecting wavelength was 350 nm,the column was 25 ℃,and the injection volume was 10 μL.Results:The calibration curve was linear in the range of 0.09-0.91 μg of luteolin-7-O-glucoside with correlation coefficient of 0.9999;The average recovery(n=6) was 103.1% with RSD of 2.1%;The limit of detection(S/N=3) was 3.48 ng and the limit of quantification(S/N=10) was 8.70 ng.Conclusion:This method is accurate,and can be used to determine the content of luteolin-7-O-glucoside in Flos Lonicerae Japonicae.

About this research paper

What this paper is about

Objective:To develop an HPLC method for the determination of luteolin-7-O-glucoside in Flos Lonicerae Japonicae.Method:An Agilent ZORBAX SB-phenyl column(4.6 mm×250 mm,5 μm) was adopted;The mobile phase was acetonitrile-0.5% acetic acid with gradient elution mode at a flow rate of 1 mL·min-1;The detecting wavelength was 350 nm,the column was 25 ℃,and the injection volume was 10 μL.Results:The calibration curve was linear in the range of 0.09-0.91 μg of luteolin-7-O-glucoside with correlation coefficient of 0.9999;The average recovery(n=6) was 103.1% with RSD of 2.1%;The limit of detection(S/N=3) was 3.48 ng and the limit of quantification(S/N=10) was 8.70 ng.Conclusion:This method is accurate,and can be used to determine the content of luteolin-7-O-glucoside in Flos Lonicerae Japonicae.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To develop an HPLC method for the determination of luteolin-7-O-glucoside in Flos Lonicerae Japonicae.Method:An Agilent ZORBAX SB-phenyl column(4.6 mm×250 mm,5 μm) was adopted;The mobile phase was acetonitrile-0.5% acetic acid with gradient elution mode at a flow rate of 1 mL·min-1;The detecting wavelength was 350 nm,the column was 25 ℃,and the injection volume was 10 μL.Results:The calibration curve was linear in the range of 0.09-0.91 μg of luteolin-7-O-glucoside with correlation coefficient of 0.9999;The average recovery(n=6) was 103.1% with RSD of 2.1%;The limit of detection(S/N=3) was 3.48 ng and the limit of quantification(S/N=10) was 8.70 ng.Conclusion:This method is accurate,and can be used to determine the content of luteolin-7-O-glucoside in Flos Lonicerae Japonicae.

Key concepts: Chemistry, Flos, Chromatography, Luteolin, Calibration curve, High-performance liquid chromatography, Detection limit, Glucoside

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Optimization of an HPLC method for determination of luteolin-7-O-glucoside in Flos Lonicerae Japonicae — Research Paper | ScholarLens