2011•Journal of Tianjin University of Traditional Chinese MedicineRequires access
Simultaneous determination of luteolin-7-O-glu-coside and chlorogenic acid in Flos Lonicerae Japonicae by HPLC
Tao Chen
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Abstract
[Objective] To establish a HPLC method for quantitative determination of luteolin-7-O-glu-coside and chlorogenic acid in Flos Lonicerae Japonicae using gradient elution method.[Methods] Determining column: Xterra@ RP 185;Detector: Waters 2 998 diode array detector(DAD);Workstation: Waters Empower;The mobile phase: 0.5% acetic acid-acetonitrile;Linear gradient elution: 0~15min(90 ∶ 10-80 ∶ 20),15~30 min(80 ∶ 20-75 ∶ 25),30~40 min(75 ∶ 25-65 ∶ 35),40~45 min(65 ∶ 35-90∶ 10);The flow rate was 1 mL/min,and the detection wavelength was set at 350 nm.[Results] In this chromatographic conditions,the two components were completely separated.[Conclusion] The method is simple,accurate,fast and easy,which can detect two components of Flos Lonicerae Japonicae simultaneously.