HPLC Method for the Quantitative Determination of Magnolol and Honokiol in the Huoxiang Zhengqi Wan
Zhou Yu-xin
Abstract
Zhou Yu-xin
Abstract
Objective:To establish a method for determining Magnolol and Honokiol in the Huoxiang Zhengqi Wan. Method:An HPLC method was designed, which was performed under the following conditions:Zorbax sb-C18 column (4.6mm×150mm, 5μm) as the chromatographic column, acetonitrile-water(68∶32) as the mobile phase at a flow velocity of 1.0mL/min, and the wavelength of 294nm as the detecting wavelength. Results:The calibration curves were linear in the range of 0.18μg~3.5μg magnolol and 0.11μg~2.2μg honokiol. The RSD was 1.5%(n=5) and 2.7%(n=5), and the average recovery was 98.5% and 101.8%, respectively. Conclusion:This HPLC method is easy and accurate,and can be used as one of the methods for controlling the quality of the Huoxiang Zhengqi Wan.
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Objective:To establish a method for determining Magnolol and Honokiol in the Huoxiang Zhengqi Wan. Method:An HPLC method was designed, which was performed under the following conditions:Zorbax sb-C18 column (4.6mm×150mm, 5μm) as the chromatographic column, acetonitrile-water(68∶32) as the mobile phase at a flow velocity of 1.0mL/min, and the wavelength of 294nm as the detecting wavelength. Results:The calibration curves were linear in the range of 0.18μg~3.5μg magnolol and 0.11μg~2.2μg honokiol. The RSD was 1.5%(n=5) and 2.7%(n=5), and the average recovery was 98.5% and 101.8%, respectively. Conclusion:This HPLC method is easy and accurate,and can be used as one of the methods for controlling the quality of the Huoxiang Zhengqi Wan.
Key concepts: Magnolol, Honokiol, Chromatography, High-performance liquid chromatography, Calibration curve, Chemistry, Detection limit