SPE-HPLC determination of magnolol and honokiol in Huoxiang Zhengqi solution

Objective:Based on the improvement of the sample pretreatment method in the criterion,an SPE-HPLC method for determining the contents of magnolol and honokiol in Huoxiang Zhengqi solution was established.Methods:Samples were purified by silica gel SPE column.The HPLC column used in the experiment was C18(250 mm×4.6 mm,5 μm),and the mobile phase was methanol-water-acetonitrile-glacial acetic acid(70:25:5:0.25)at the flow rate of 1.0 mL·min-1;The detection wavelength was 294 nm,and the experiment was accomplished at room temperature.Results:The contents of magnolol and honokiol determined by SPE-HPLC method had good linear relationship with the peak areas when their concentrations at 4.0-80.0 μg·mL-1(r=0.9998),2.0-40.0 μg·mL-1(r=0.9998),respectively;The average recovery(n=5)of magnolol was 99.9%(RSD=0.7%),and that of honokiol was 99.5%(RSD=0.5%).Conclusion:The method is environmental protection,simple,accurate,with good reproducibility,and it can be used for the quality control of Huoxiang Zhengqi solution.