Combined Detection of P504S,p63 and 34βE12 in Pathological Diagnosis of Prostatic Carcinoma

Objective To investigate the value of the combined detection of P504S,p63 and 34βE12 in the early and differential diagnosis of prostatic carcinoma.Methods Immunohistochemical staining EnVision method was used to detect the expression of P504S,p63 and 34βE12 in 38 cases of prostatic carcinoma(PCa),9 cases of high-grade prostatic intraepithelial neoplasia(HGPIN),5 cases of atypical adenomatous hyperplasia(AAH),11 cases of basal cell hyperplasia(BCH) and 32 cases of benign prostatic hyperplasia(BPH).Results The positive expression rates of P504S in PCa,HGPIN,AAH,BCH and BPH were 94.7%,88.9%,20.0%,0 and 9.4% respectively,and the expression rate in PCa,HGPIN was higher than that in AAH,BCH and BPH(P0.05).p63 and 34βE12 were negative in all cases of PCa,and were positive in all cases of HGPIN,AAH,BCH and BPH,and the expression rate in PCa was lower than that in HGPIN,AAH,BCH and BPH(P0.01).The level of expression of P504S was not correlated with PCa Gleason score(r=-0.035,P=0.833).Conclusion Combined detection of P504S,p63 and 34βE12 can be most helpful for pathological diagnosis of PCa and improve the detection rate of small local PCa,and it has a great significance in the early and differential diagnosis of PCa,especially in needle biopsy specimen