Application of CK34βE12,p63 and P504S in diagnosis of prostate cancer

Objective To evaluate the value of CK34βE12,p63 and P504S immunostaining in diag- nosis of benign and malignant lesions of the prostate.Methods Expression of CK34βE12,p63 and P504S in 74 benign and malignant lesions of prostate,including 27 PC,6 HGPIN,10 LGPIN,3 AAH and 28 BPH were observed by immunohistochemical method.Results Expression of p63 and CK34βE12 were positive in AAH,LGPIN and BPH,all of PC were negative,the positive rate of HGPIN was 83.3%(5/6).There were sig- nificant differences in p63 and CK34βE12 expression between PC and AAH,HGPIN,LGPIN,BPH(P0.01). The P504S positive rates in PC,AAH,HGPIN,LGPIN and BPH were 92.6 %(25127),0,66.7 %(4/6),10(1/ 10)and 0.The positive rate in PC was significantly higher than that of AAH,LGPIN and BPH(P0.01). There was no significant difference between PC and HGPIN(P=0.132).The P504S positive expression com- bined with the p63 or CK34βE12 negative expression was recognized in PC,AAH,HGPIN,LGPIN and BPH at the rates of 92.6 %,0,16.7 %,0 and 0,respectively.There were significant differences between PC and AAH,HGPIN,LGPIN,BPH(P0.01).Conclusion Combined detection of P504S and p63 or CK34βE12 can be most helpful for the diagnosis of prostate carcinoma.