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Determination of flavonoids in Folium Mori

Li Huang

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Abstract

Objective:To determine the contents of flavonoids(rutin and quercitrin )in Folium Mori from different sources.Method :The determination was performed by HPLC,using Merck RP-C_(18)( 250mm×4.6 mm,5μm), methanol-0.5%phosphoric acid gradient elution as mobile phase.The flow rate was set at 1.0 mL·min~(-1)and the column temperature was set at 25℃.the wavelength of ditector was 358 nm.Result:The result of the content determination shows that rutin and quercitrin have good linear relation from 0.10 μg to 1.01μg and 0.06 μg to 0.60μg respectively.Conclusion:The results show the method above is accurate,rapid and reproducibility,which would be beneficial for the quality control of this drug.

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What this paper is about

Objective:To determine the contents of flavonoids(rutin and quercitrin )in Folium Mori from different sources.Method :The determination was performed by HPLC,using Merck RP-C_(18)( 250mm×4.6 mm,5μm), methanol-0.5%phosphoric acid gradient elution as mobile phase.The flow rate was set at 1.0 mL·min~(-1)and the column temperature was set at 25℃.the wavelength of ditector was 358 nm.Result:The result of the content determination shows that rutin and quercitrin have good linear relation from 0.10 μg to 1.01μg and 0.06 μg to 0.60μg respectively.Conclusion:The results show the method above is accurate,rapid and reproducibility,which would be beneficial for the quality control of this drug.

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Available abstract

Objective:To determine the contents of flavonoids(rutin and quercitrin )in Folium Mori from different sources.Method :The determination was performed by HPLC,using Merck RP-C_(18)( 250mm×4.6 mm,5μm), methanol-0.5%phosphoric acid gradient elution as mobile phase.The flow rate was set at 1.0 mL·min~(-1)and the column temperature was set at 25℃.the wavelength of ditector was 358 nm.Result:The result of the content determination shows that rutin and quercitrin have good linear relation from 0.10 μg to 1.01μg and 0.06 μg to 0.60μg respectively.Conclusion:The results show the method above is accurate,rapid and reproducibility,which would be beneficial for the quality control of this drug.

Key concepts: Quercitrin, Chemistry, Rutin, Folium of Descartes, Chromatography, Phosphoric acid, Gradient elution, Flavonoid

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