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Content determination of quercetin in Folium Loropetali

Xiaojiang Zhou

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Abstract

Objective To study a RP-HPLC method for the content determination of Quercetin in Folium Loropetali.Methods The C18 column,mixture of acetonitrile and 0.4% phosphoric acid(32:68)as mobile phase and UV detection at 360 nm were used for the determination.Results Quercetin was better in linear correlation between 0.06～0.62 μg(r=0.999 9),the recovery rate was 98.18%,RSD=1.97%.Conclusion The method is very stable and accurate and has good reappearance.which can be used for quality control of Folium Loropetali.

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What this paper is about

Objective To study a RP-HPLC method for the content determination of Quercetin in Folium Loropetali.Methods The C18 column,mixture of acetonitrile and 0.4% phosphoric acid(32:68)as mobile phase and UV detection at 360 nm were used for the determination.Results Quercetin was better in linear correlation between 0.06～0.62 μg(r=0.999 9),the recovery rate was 98.18%,RSD=1.97%.Conclusion The method is very stable and accurate and has good reappearance.which can be used for quality control of Folium Loropetali.

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Available abstract

Objective To study a RP-HPLC method for the content determination of Quercetin in Folium Loropetali.Methods The C18 column,mixture of acetonitrile and 0.4% phosphoric acid(32:68)as mobile phase and UV detection at 360 nm were used for the determination.Results Quercetin was better in linear correlation between 0.06～0.62 μg(r=0.999 9),the recovery rate was 98.18%,RSD=1.97%.Conclusion The method is very stable and accurate and has good reappearance.which can be used for quality control of Folium Loropetali.

Key concepts: Folium of Descartes, Quercetin, Phosphoric acid, Chemistry, Chromatography, High-performance liquid chromatography, Content determination, Biochemistry

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