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Determination of Rutin and Quercetin in Folium Apocyni Venety by RP-HPLC

Chuan Feng

Open publisher page 1 citations

Abstract

Objective:To develop an HPLC method for determination of rutin and quercetin in Folium apocyni venety.Methods:Analyses were carried out using a kromasil C18 column(4.6 mm×250 mm,5μm).The mobile phase was A(methanol)-B(0.08% phosphoric acid solution)with gradient elution and the flow rate was 1.0mL/min,the UV detector was set at 360nm.Results:A linear relationship of rutin was good at the range of 0.42~7.10μg(r=0.9998)and quercetin was good at the range of 0.011~0.19μg(r=0.9997).The average recovery of rutin was 98.77%(RSD%=1.2) and of quercetin was 99.19%(RSD%=1.8).Conclusion:The method is simple,accurate and good reproducibility.It is suitable for the simultaneous determination of rutin and quercetin in Folium apocyni venety.

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Objective:To develop an HPLC method for determination of rutin and quercetin in Folium apocyni venety.Methods:Analyses were carried out using a kromasil C18 column(4.6 mm×250 mm,5μm).The mobile phase was A(methanol)-B(0.08% phosphoric acid solution)with gradient elution and the flow rate was 1.0mL/min,the UV detector was set at 360nm.Results:A linear relationship of rutin was good at the range of 0.42~7.10μg(r=0.9998)and quercetin was good at the range of 0.011~0.19μg(r=0.9997).The average recovery of rutin was 98.77%(RSD%=1.2) and of quercetin was 99.19%(RSD%=1.8).Conclusion:The method is simple,accurate and good reproducibility.It is suitable for the simultaneous determination of rutin and quercetin in Folium apocyni venety.

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Available abstract

Objective:To develop an HPLC method for determination of rutin and quercetin in Folium apocyni venety.Methods:Analyses were carried out using a kromasil C18 column(4.6 mm×250 mm,5μm).The mobile phase was A(methanol)-B(0.08% phosphoric acid solution)with gradient elution and the flow rate was 1.0mL/min,the UV detector was set at 360nm.Results:A linear relationship of rutin was good at the range of 0.42~7.10μg(r=0.9998)and quercetin was good at the range of 0.011~0.19μg(r=0.9997).The average recovery of rutin was 98.77%(RSD%=1.2) and of quercetin was 99.19%(RSD%=1.8).Conclusion:The method is simple,accurate and good reproducibility.It is suitable for the simultaneous determination of rutin and quercetin in Folium apocyni venety.

Key concepts: Rutin, Folium of Descartes, Quercetin, Chromatography, Phosphoric acid, Chemistry, High-performance liquid chromatography, Linear range

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