Simultaneous Determination of Chlorogenic Acid,Rutin,Isoquercetin,Astragalin and Quercetin in Mori Folium by HPLC

Objective: To establish HPLC method for simultaneous determination of chlorogenic acid,rutin,isoquercetin and astragalin quercetin in Mori Folium. Method: A HPLC equipped with a Thermo ODS( 4. 6 mm ×250 mm,5 μm) column and UV detection was used. The column temperature was at 35 ℃ and the flow rate 1. 0 mL·min- 1. The mobile phase was consisited of phosphoric acid in acetonitrile( 0. 1%,A) and phosphoric acid( 0. 16%) and triethylamine( 0. 18%) in water( B) with gradient elution. The detection wavelength was set at 360 nm. Result: The good saperation of five flavo noids was achieved within 60 min. Linear calibration curves were obtained with 0. 32-13. 73 μg( r = 0. 999 6),0. 05-3. 13 μg( r = 0. 999 8),0. 03-9. 50μg( r = 0. 999 4),0. 03-5. 60 μg( r = 0. 999 5),0. 001-0. 05 μg( r = 0. 999 7); the average recovery was99. 61%-106. 09%,RSD≤2. 8%. Conclusion: The proposed method is simple,accurate,repeatable,and can be used for quality control as well as a powerful tool to evaluate the processing of Mori Folium.