Cloning and function analysis of promoter of heat shock protein 81.1 from Brassica napus L.

Promoter of temperature-induced heat shock protein(HSP) gene is used to control the exogenous gene expression not only to improve the expression accuracy,but also to enhance the safety of GM(genetically modified) crop.In present study,a HSP gene promoter from Brassica napus L.was cloned onto the upstream of GUS reporter gene in binary vector pBI121,in order to construct a binary expression vector pBI121 HSP GUS.The vector was then transformed into tobacco by Agrobacterium tumefaciens-mediated method.It was shown that T1 plants started to express the foreign GUS gene at 42℃ induction for 1h.The expression rate and amount of GUS gene varied at different growth stages and reached to a high level around the 50th day after seeding.Then the expression decreased gradually.The results indicated a potential of HSP promoter from rapeseed to control the expression of tobacco target gene under heat condition.