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2001•Acta Photophysiologica SinicaRequires access

Expression of GUS Gene in Transgenic Tobacco Regulated by BcNA1 Promoter from Brassica napus

Shi Dong

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Abstract

The upstream regulatory region of a seed specific gene was separated from Brassica napus cv. H165 genomic DNA by polymerase chain reaction. Plant expression vector was constructed by ligating this promoter with GUS gene and transferred into tobacco. GUS analysis made on the transgenic plants indicated that the promoter could drive the expression of GUS gene seed specifically and the activity of GUS changed with the development of seeds. The results of GUS assay also suggested that the genetic transmission of the BcNA1 promoter conforms to Mendelian laws.

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What this paper is about

The upstream regulatory region of a seed specific gene was separated from Brassica napus cv. H165 genomic DNA by polymerase chain reaction. Plant expression vector was constructed by ligating this promoter with GUS gene and transferred into tobacco. GUS analysis made on the transgenic plants indicated that the promoter could drive the expression of GUS gene seed specifically and the activity of GUS changed with the development of seeds. The results of GUS assay also suggested that the genetic transmission of the BcNA1 promoter conforms to Mendelian laws.

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Available abstract

The upstream regulatory region of a seed specific gene was separated from Brassica napus cv. H165 genomic DNA by polymerase chain reaction. Plant expression vector was constructed by ligating this promoter with GUS gene and transferred into tobacco. GUS analysis made on the transgenic plants indicated that the promoter could drive the expression of GUS gene seed specifically and the activity of GUS changed with the development of seeds. The results of GUS assay also suggested that the genetic transmission of the BcNA1 promoter conforms to Mendelian laws.

Key concepts: Brassica, Gene, Biology, GUS reporter system, Transgene, Promoter, Genetically modified crops, Genetics

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Expression of GUS Gene in Transgenic Tobacco Regulated by BcNA1 Promoter from Brassica napus — Research Paper | ScholarLens