Cloning,Sequence Analysis and Transient Expression of BnGOLS1 Gene Promoter from Brassica napus

The BnGOLS1 promoter fragment(827 bp) was amplified from the genomic DNA of Brassica napus by inverse polymerase chain reaction.Promoter sequence analysis by PLACE and PlantCARE showed that the cloned fragment contained several putative cis-elements,such as abscisic acid response element(ABRE),dehydration-responsive element(DRE),heat shock elements(HSE),WRKY transcription factor recognition site w-Box and so on,as well as TATA-Box and CAAT-Box.A recombinant vector designated as pBI-GS-GUS was generated through the replacement of CaMV35S promoter in pBI121 by the cloned BnGOLS1 promoter fragment,in which the reporter GUS is under the control of BnGOLS1 promoter.Transient expression of pBI-GS-GUS in Brassica napus was performed by Agrobacterium tumefaciens mediated method.Histochemical staining of GUS revealed that the promoter of BnGOLS1 could drive the expression of GUS gene in Brassica napus.