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2013•Zhongguo youliao zuowu xuebaoRequires access

Cloning and function analysis of promoter of RabGDI3 from Brassica napus L.

Xuanpeng Wang, Zhenbo Li, Tong Chaobo, Huang Jun-yan, Jingyin Yu, Dong Cai-hua, Liu S

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Abstract

Bioinformation showed that BnRabGDI3 gene promoter included a 2 064bp-long segment at the upstream translation start site in Brassica napus genome.Promoter sequence contained several anther specific cis-elements.To verify the biology function,a recombinant vector was designated as pBnRabGDI3:GUS through the replacement of CaMV35S promoter in pBI121 by cloned BnRabGDI3 promoter fragment.In the vector,gus reporter gene was driven by BnRabGDI3 promoter.GUS histochemical assay in six independent transgenic plants showed that gus expressed only in anthers from the 11th to 13th development stages.The highest level of GUS activity was detected in bicellular and tricellar microspores,suggesting BnRabGDI3 played an important role in microspore development.

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What this paper is about

Bioinformation showed that BnRabGDI3 gene promoter included a 2 064bp-long segment at the upstream translation start site in Brassica napus genome.Promoter sequence contained several anther specific cis-elements.To verify the biology function,a recombinant vector was designated as pBnRabGDI3:GUS through the replacement of CaMV35S promoter in pBI121 by cloned BnRabGDI3 promoter fragment.In the vector,gus reporter gene was driven by BnRabGDI3 promoter.GUS histochemical assay in six independent transgenic plants showed that gus expressed only in anthers from the 11th to 13th development stages.The highest level of GUS activity was detected in bicellular and tricellar microspores,suggesting BnRabGDI3 played an important role in microspore development.

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Available abstract

Bioinformation showed that BnRabGDI3 gene promoter included a 2 064bp-long segment at the upstream translation start site in Brassica napus genome.Promoter sequence contained several anther specific cis-elements.To verify the biology function,a recombinant vector was designated as pBnRabGDI3:GUS through the replacement of CaMV35S promoter in pBI121 by cloned BnRabGDI3 promoter fragment.In the vector,gus reporter gene was driven by BnRabGDI3 promoter.GUS histochemical assay in six independent transgenic plants showed that gus expressed only in anthers from the 11th to 13th development stages.The highest level of GUS activity was detected in bicellular and tricellar microspores,suggesting BnRabGDI3 played an important role in microspore development.

Key concepts: Microspore, GUS reporter system, Biology, Gene, Promoter, Cloning (programming), Reporter gene, Genetics

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Cloning and function analysis of promoter of RabGDI3 from Brassica napus L. — Research Paper | ScholarLens