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2009•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Chlorogenic Acid in Fufangshuanghuakeli by HPLC

Ling Hai

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Abstract

Aim To establish an assaying method of chlorogenic acid in Fufangshuanghuakeli.Methods HPLC PhenomenexC18 analytical column(5μm,4.6mm×250mm)was used and acetonitrile-0.4 phosphonic acid(10∶90,pH value was adjusted to 3.0±0.2 by triethylamine)was used as the mobile phase.The flow rate was 1.0mL/min and the detection wavelength was 327nm.Results Regression equation of chlorogenic acid was Y=3 621.370 1X+2.145 8,r=0.999 9(n=5).It showed linear correlation when concentration of chlorogenic acid ranged from 12.0μg·mL-1 to 28.0μg·mL-1.The average recovery was 98.53% and RSD was 0.57%.Conclusion This method is convenient,accurate and rapid,showing its potential to be used as a quantitative method of this preparation.

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Aim To establish an assaying method of chlorogenic acid in Fufangshuanghuakeli.Methods HPLC PhenomenexC18 analytical column(5μm,4.6mm×250mm)was used and acetonitrile-0.4 phosphonic acid(10∶90,pH value was adjusted to 3.0±0.2 by triethylamine)was used as the mobile phase.The flow rate was 1.0mL/min and the detection wavelength was 327nm.Results Regression equation of chlorogenic acid was Y=3 621.370 1X+2.145 8,r=0.999 9(n=5).It showed linear correlation when concentration of chlorogenic acid ranged from 12.0μg·mL-1 to 28.0μg·mL-1.The average recovery was 98.53% and RSD was 0.57%.Conclusion This method is convenient,accurate and rapid,showing its potential to be used as a quantitative method of this preparation.

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Available abstract

Aim To establish an assaying method of chlorogenic acid in Fufangshuanghuakeli.Methods HPLC PhenomenexC18 analytical column(5μm,4.6mm×250mm)was used and acetonitrile-0.4 phosphonic acid(10∶90,pH value was adjusted to 3.0±0.2 by triethylamine)was used as the mobile phase.The flow rate was 1.0mL/min and the detection wavelength was 327nm.Results Regression equation of chlorogenic acid was Y=3 621.370 1X+2.145 8,r=0.999 9(n=5).It showed linear correlation when concentration of chlorogenic acid ranged from 12.0μg·mL-1 to 28.0μg·mL-1.The average recovery was 98.53% and RSD was 0.57%.Conclusion This method is convenient,accurate and rapid,showing its potential to be used as a quantitative method of this preparation.

Key concepts: Chlorogenic acid, Triethylamine, Chromatography, High-performance liquid chromatography, Chemistry, Acetonitrile, Linear regression, Linear relationship

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