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Determination of Chlorogenic acid in Xiaoeryanbiankeli by HPLC

Jiajia Liu

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Abstract

Objective:To establish a method for the determination of Chlorogenic acid in Xiaoeryanbiankeli by HPLC.Methods:We used the Hypersil ODS chromatographic column(4.6mm×250mm×5μm) with the mobile phase consisting of a mixture of acetonitrile:0.4% phosphoric acid(10:90).The flow rate was at 0.8mL/min,and the UV detection wavelength was at 327nm and the column temperature was at 25℃.Results:The linear range of chlorogenic acid was at the range of 0.034μg in 0.34μg(r=0.9998).The average recovery was 98.7%,and RSD was 2.29%(n=5).Conclusion:The established method is simple,accurate and rapid with a good reproducibility and can be used for the quality control of Xiaoeryanbiankeli.

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What this paper is about

Objective:To establish a method for the determination of Chlorogenic acid in Xiaoeryanbiankeli by HPLC.Methods:We used the Hypersil ODS chromatographic column(4.6mm×250mm×5μm) with the mobile phase consisting of a mixture of acetonitrile:0.4% phosphoric acid(10:90).The flow rate was at 0.8mL/min,and the UV detection wavelength was at 327nm and the column temperature was at 25℃.Results:The linear range of chlorogenic acid was at the range of 0.034μg in 0.34μg(r=0.9998).The average recovery was 98.7%,and RSD was 2.29%(n=5).Conclusion:The established method is simple,accurate and rapid with a good reproducibility and can be used for the quality control of Xiaoeryanbiankeli.

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Available abstract

Objective:To establish a method for the determination of Chlorogenic acid in Xiaoeryanbiankeli by HPLC.Methods:We used the Hypersil ODS chromatographic column(4.6mm×250mm×5μm) with the mobile phase consisting of a mixture of acetonitrile:0.4% phosphoric acid(10:90).The flow rate was at 0.8mL/min,and the UV detection wavelength was at 327nm and the column temperature was at 25℃.Results:The linear range of chlorogenic acid was at the range of 0.034μg in 0.34μg(r=0.9998).The average recovery was 98.7%,and RSD was 2.29%(n=5).Conclusion:The established method is simple,accurate and rapid with a good reproducibility and can be used for the quality control of Xiaoeryanbiankeli.

Key concepts: Chlorogenic acid, Chromatography, Phosphoric acid, High-performance liquid chromatography, Reproducibility, Chemistry, Acetonitrile, Linear relationship

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