Purification Technology of Total Flavonoids from Carthamus tinctorius by Macroporous Resin

Objective:To study on purification technology conditions of total flavonoids from Carthamus tinctorius by macroporous resin.Method: Absorption rate and desorption rate of total flavonoids were taken as indexes,investigated absorption and desorption ability of 6 different types macroporous resin to safflower flavonoids,determined AB-8 was optimum macroporous resin.Purification conditions of AB-8 macroporous resin were optimized.Result:Optimum technology conditions were as follows:liquid concentration was 50 g·L-1(pH 2),flow rate of sample was 4 BV·h-1;eluted with 3 BV the amount of 30% ethanol by speed of 2 BV·h-1.After purified with AB-8 macroporons resin,purity of total flavonoids was up to 54.04%,purity of hydroxysafflor yellow A was up to 28.76%,separation effect was good.Conclusion: AB-8 resin was fit for separating and purifying of total flavonoids from safflower.