Study on separation and purification process of total flavonoids of safflower by macroporous resins

The characteristic of Absorption and adsorption of four kinds of macroporous resins to extract flavonoids of safflower was studied.Suitable resin for purification of flavonoids was screened from four resins,and its characteristic of Absorption and adsorption was investigated,through single factor experiments.The results indicated that AB-8 macroporous resins had better Absorption and adsorption property for purification of flavonoids from safflower.The optimum Absorption conditions of macroporous resin AB-8 was as follows:resin to sample 1:10(g/mL),sample concentration 3mg/mL,the value of pH4.7,the time of Absorption and desorption was 6h and 4h.The optimum dynamic adsorption was obtained with the flow rate 2BV/h,sample concentration 3mg/mL,and 70% alcohol as eluant for desorption with the flow rate 2BV/h.63.31% purity can be separated from crude safflower extract(about 21.85% purity)with AB-8 resin by the following optimum dynamic adsorption and desorption conditions.