Optimization of Purification Process for Total Flavonoids from Leaves of Eriobotrya japonica by Macroporous Resin

Objective:To optimize enrichment technology conditions of total flavonoids from leaves of Eriobotrya japonica by macroporous adsorption resin.Method: With rutin as reference substance,the content of total flavonoids was determined by aluminium nitrate chromogenic method.With static adsorption capacity and static desorption rate as indexes,optimum type of macroporous resin was selected by comparing adsorption and desorption effect of 6 kinds macroporous resin for total flavonoids from leaves of E.japonica,and to investigate its absorption and desorption conditions.Result: HPD 100 macroporous resin was the most suitable for purification of total flavonoids from E.japonica.Optimum purification conditions were:the concentration of sampling liquid of 3 g·L-1,sampling velocity 2 BV·h-1,sample liquid volume 2.5 BV,eluted by 2 BV deionized water and 5 BV 70% ethanol at the speed of 1 BV·h-1,eluate was collected.Under these process conditions,yield of total flavonoids was 78.7%,purity of total flavonoids was 47.3%.Conclusion: HPD 100 macroporous resin had a good purification effect of total flavonoids from leaves of E.japonica,optimized purification process was reasonable,stable and feasible.