2013•Zhongguo shiyan fangjixue zazhiRequires access
Optimization of Purification Technology for Total Flavonoids in Angelica pubescens by Macroporous Adsorption Resin
Shuai Chen
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Abstract
Objective:To optimize purification technology of total flavonoids in Angelica pubescens by macroporous resin.Method:With adsorption rate and desorption rate of total flavonoids as indexes,macroporous resin models were optimized by static adsorption and desorption experiments as well as dynamic adsorption and desorption tests;Purification technology parameters of total flavonoids were optimized by single factor test.Result:LSA-33 macroporous resin presented the best effect of purification,optimum purification conditions were as followings:the concentration of total flavonoids from sample solution 0.1 g·mL-1,pH of sample solution 2-3,adsorption rate 4 BV·h-1,eluted with 3 BV 75% ethanol,desorption rate 3 BV·h-1,collected eluent,purity of total flavonoids in dry extract of A.pubescens was changed from 9.87% to 32.59%.Conclusion:LSA-33 macroporous resin was suitable for preliminary purification of total flavonoids in A.pubescens.