2010•Medical Journal of National Defending Forces in North ChinaRequires access
Determination of Paeonol Contents in Cortex Moutan by High Performance Liquid Chromatography (HPLC) from Different Habitats and Different Years
Xiao‐Song Li
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Abstract
Objective To establish a HPLC method for determination of paeonol in Cortex Moutan from different habitats and different years.Methods The column methods were Diamonsil C18(4.6 mm×200.0 mm,5.0 μm).The mobile phase was a combination of me-thanol,aceticacid and water in the volume ratio of 54:2:44 at the flow rate of 1 ml/min.The elvation was monitored with UV absorption at 274 nm.The system worked at room temperater.Results The lineal range of Paeonol were 5.1-102.0 μg/ml,r= 0.9996,the mean recovery was 99.4%,and RSD was 0.94%.There was significant difference in contents of paeonol in Cortex Moutan from different habitats and different years(P0.01).Conclusion This method is accurate,simple and reliable and can be used to control quality of Cortex Moutan.