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2009•Journal of Anhui Traditional Chinese Medical CollegeRequires access

Content Determination of Paeonol in Compound Cortex Moutan Decoction by High Performance Liquid Chromatography

Deling Wu

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Abstract

Objective:To determine the content of paeonol in Compound Cortex Moutan Decoction by high performance liquid chromatography(HPLC) method.Methods: The samples were analyzed by a VP-ODS column,with mobile phase methanol-water(50∶50) at flow rate of 1.00 ml/min,detection at wavelength of 274 nm and the temperature at 30 ℃.Results: The calibration curves of paeonol were in good linearity over the range of 5.36～26.8 μg/ml(r=0.999 7).The average recovery rate was 99.12%(n=5) and RSD was 1.25%.Conclusion: The HPLC method is simple,accurate and reproducible,and it can be used for the quality control standard of paeonol in Compound Cortex Moutan Decoction.

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Objective:To determine the content of paeonol in Compound Cortex Moutan Decoction by high performance liquid chromatography(HPLC) method.Methods: The samples were analyzed by a VP-ODS column,with mobile phase methanol-water(50∶50) at flow rate of 1.00 ml/min,detection at wavelength of 274 nm and the temperature at 30 ℃.Results: The calibration curves of paeonol were in good linearity over the range of 5.36～26.8 μg/ml(r=0.999 7).The average recovery rate was 99.12%(n=5) and RSD was 1.25%.Conclusion: The HPLC method is simple,accurate and reproducible,and it can be used for the quality control standard of paeonol in Compound Cortex Moutan Decoction.

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Available abstract

Objective:To determine the content of paeonol in Compound Cortex Moutan Decoction by high performance liquid chromatography(HPLC) method.Methods: The samples were analyzed by a VP-ODS column,with mobile phase methanol-water(50∶50) at flow rate of 1.00 ml/min,detection at wavelength of 274 nm and the temperature at 30 ℃.Results: The calibration curves of paeonol were in good linearity over the range of 5.36～26.8 μg/ml(r=0.999 7).The average recovery rate was 99.12%(n=5) and RSD was 1.25%.Conclusion: The HPLC method is simple,accurate and reproducible,and it can be used for the quality control standard of paeonol in Compound Cortex Moutan Decoction.

Key concepts: Paeonol, Chromatography, High-performance liquid chromatography, Chemistry, Decoction, Paeoniflorin, Paeonia suffruticosa, Content determination

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Content Determination of Paeonol in Compound Cortex Moutan Decoction by High Performance Liquid Chromatography — Research Paper | ScholarLens