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Determination of Paeonol in Cortex Moutan by HPLC

Jie Zhao

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Abstract

To determine paeonol in cortex moutan from five areas by HPLC.HPLC column was a Phenomenex Luna C18(5μm,4.6mm×250 mm),with mobile phase of methanol-water(45∶55) and the flow rate at 1 mL/min.The detective wavelength was 274 nm and the column temperature was 25℃.The results showed that the linear of paeonol was good in the range of 0.432μg—4.32 μg(r=0.9997),and the average recovery was 100.08%(RSD=2.85%).The average content of paeonol in cortex moutan was: AnhuiShanxiShandongHenanChongqing.This method was rapid and convenient,results were correct and reliable.

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What this paper is about

To determine paeonol in cortex moutan from five areas by HPLC.HPLC column was a Phenomenex Luna C18(5μm,4.6mm×250 mm),with mobile phase of methanol-water(45∶55) and the flow rate at 1 mL/min.The detective wavelength was 274 nm and the column temperature was 25℃.The results showed that the linear of paeonol was good in the range of 0.432μg—4.32 μg(r=0.9997),and the average recovery was 100.08%(RSD=2.85%).The average content of paeonol in cortex moutan was: AnhuiShanxiShandongHenanChongqing.This method was rapid and convenient,results were correct and reliable.

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Available abstract

To determine paeonol in cortex moutan from five areas by HPLC.HPLC column was a Phenomenex Luna C18(5μm,4.6mm×250 mm),with mobile phase of methanol-water(45∶55) and the flow rate at 1 mL/min.The detective wavelength was 274 nm and the column temperature was 25℃.The results showed that the linear of paeonol was good in the range of 0.432μg—4.32 μg(r=0.9997),and the average recovery was 100.08%(RSD=2.85%).The average content of paeonol in cortex moutan was: AnhuiShanxiShandongHenanChongqing.This method was rapid and convenient,results were correct and reliable.

Key concepts: Paeonol, Chromatography, High-performance liquid chromatography, Chemistry, Cortex (anatomy), Medicine, Biology, Alternative medicine

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