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[Optimization of extraction parameters and de-protein process for Gastrodia elata. Polysaccharides].

Xiaoxia Zhu, Xuegang Luo

Abstract

OBJECTIVE: Grope for Optimization of Extraction Parameters and De-protein Process for Gastrodia elata Bl. Polysaccharides (GPS). Provide the theoretical basis for the industrialization production of GPS. METHODS: The effects of extracting temperature, ration between Gastrodia elata Bl. and water, heating time on extraction rate of GPS were studied with orthogonal experimental design. Compared the application of the two de-protein process measures (Sevag and Enzyme-Sevag) in purification technics for Gastrodia elata Bl. RESULTS: The optimum parameters were: extraction 3h at 120 degrees C with 1:40 ratio of Gastrodia elata Bl. to water. The de-protein efficiency of Enzyme-Sevag is better than Sevag.

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Key concepts: Gastrodia elata, Extraction (chemistry), Polysaccharide, Chromatography, Chemistry, Biochemistry, Medicine, Pathology