Optimization of Extraction Parameters for Polysaccharides of Gastrodia elata Bl.

Objective To optimize the extraction parameters and experimentation pretreatments for Polysaccharides Gastrodia elata Bl.(GPS).Methods The effects of extracting temperature,ratio between Gastrodia elata Bl.and water,heating time on extraction rate of GPS were studied with orthogonal experimental design.Compared the application of the two experimentation pretreatment(ultrasound and microwave extraction) in purification technologies for Gastrodia elata Bl..Results The extraction rate of GPS was affected obviously in the successive order of temperature,heating time,and ratio between Gastrodia elata Bl.The optimum parameters were as follows∶extraction 3 h at 120℃ with 1∶40 ratio of Gastrodia elata Bl.to water,and microwave treatment 8 min.Conclusion The methods are proved to be simple and scientific.It can be used for the quality control of GPS preparations.