The Diagnosis and the Clinical Significance of Combined Detection of ANA,Anti-dsDNA,and anti-ENA Antibody in Systematic Lupus Erthematosus

Objective To investigate the diagnostic significance of combined determination of the anti-nuclear antibody (ANA),anti-dsDNA,anti-extractable nuclear antigens(ENA) antibody in systematic lupus erythematosus.Methods Ninety-two SLE patients (SLE group) and 80 healthy people (control group)were examined and compared.ANA was tested by indirect immunofluorescence;anti-dsDNA was tested by immofiltration assay;anti-ENA antibody was tested by immunoblotting technique.Results ①The positive rate of ANA,anti-dsDNA and anti-ENA antibody in the SLE group patients were 89.1%,47.8% and 50.0% respectively;the positive rate of combined determination of ANA,anti-dsDNA and anti-ENA antibody was 94.5%.All the data in SLE group were higher than those in control group,which showed significant differences(P0.01).②The respective antibody positive rates of anti-ENA antibody in SLE group patients:the anti-Sm was 22.8%,the anti-SSA was 26.1%,the anti-SSB was 10.9%,the anti-U1RNP was 15.2%,the anti-rRNP was 9.7%,the anti-scl-70 was 1.1% and anti-Jo-1 was zero.③Of SLE group patients,40 were lupus nephtritis (LN group),52 were not lupus nephtritis(NLN group);the anti-dsDNA of the 30 patients in LN group was positive,whose rate was 75.0%;the 14 patients in NLN group are positive,whose rate is 26.9%,which shows significant differences(P0.01).Conclusion The determination of ANA,anti-dsDNA,and anti-ENA antibody may have important significance in diagnosis,differentiation,monitoring and treatment.The combined determination of ANA,anti-dsDNA and anti-ENA antibody could improve the determination percentage in SLE,which is complementary with each other.