Significance of detection of ANA,anti-ENA,anti-dsDNA natibody in diagnosis of SLE

Aim To investigate the significane of detection of anti-nuclear antibody(ANA),anti-extractable-antigen antibody(anti-ENA)and the anti-double strand DNA antibody in diagnosis of systemic erythematosus lupus(SLE). Methods ANA was detected with indirect immunity fluorescence test,the anti-ds-DNA was detected with immunogold assay,anti-ENA,anti-Sm,anti-nRNP/Sm,anti-SS-A,anti-SS-B,anti-Scl-70,anti-Jo-1 were detected with.Western blot. Results The positive rates of ANA,anti-ds-DNA,anti-Sm,anti-nRNP/Sm,anti-SS-A,anti-SS-B,anti-Scl-70 and anti-Jo -1 were 70.8%,47.5%,37.5%,29.16%26.66%,21.66%,1.66%and 0%in SLE patiente.While only ANA was positive in the control group with a positive rate of 6.67%.Conclusion ANA,anti-ds-DNA and anti-Sm can be taken as the indicator antibodies in diagnosis of SLE and joint detection can enhance diagnositc rate in addition to valuable for guidance of treatment,tracing and prognosis of the disease.