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2014•Yiyao daobaoRequires access

Content Determination of Crocin-I and Crocin-II in Chongcao Baoyuan Capsules

LI Lan-r

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Abstract

Objective To establish a HPLC method for the content determination of crocin-Ⅰ and crocin-Ⅱ in chongcao baoyuan capsules. Methods The HPLC method was carried out on a Hypersil ODS2 C18column( 150 mm × 4. 6 mm, 5 μm),with methanol-water( 48∶ 52) as the mobile phase,using the detection wavelength of 440 nm and the flow rate of 1. 0 mL·min-1. Results The calibration curves of crocin-Ⅰ and crocin-Ⅱ were linear in the ranges of 6-45 μg·mL-1( r = 1. 000 0) and 3. 2-24 μg·mL-1( r =1. 000 0),respectively. The average recoveries were 98. 73%( RSD =1. 24%,n =6) for crocin-Ⅰ and 98. 01 %( RSD =1. 53%,n =6) for crocin-Ⅱ. Conclusion The method is simple,accurate and reproducible,which is suitible for the quality control of chongcao baoyuan capsules.

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Objective To establish a HPLC method for the content determination of crocin-Ⅰ and crocin-Ⅱ in chongcao baoyuan capsules. Methods The HPLC method was carried out on a Hypersil ODS2 C18column( 150 mm × 4. 6 mm, 5 μm),with methanol-water( 48∶ 52) as the mobile phase,using the detection wavelength of 440 nm and the flow rate of 1. 0 mL·min-1. Results The calibration curves of crocin-Ⅰ and crocin-Ⅱ were linear in the ranges of 6-45 μg·mL-1( r = 1. 000 0) and 3. 2-24 μg·mL-1( r =1. 000 0),respectively. The average recoveries were 98. 73%( RSD =1. 24%,n =6) for crocin-Ⅰ and 98. 01 %( RSD =1. 53%,n =6) for crocin-Ⅱ. Conclusion The method is simple,accurate and reproducible,which is suitible for the quality control of chongcao baoyuan capsules.

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Available abstract

Objective To establish a HPLC method for the content determination of crocin-Ⅰ and crocin-Ⅱ in chongcao baoyuan capsules. Methods The HPLC method was carried out on a Hypersil ODS2 C18column( 150 mm × 4. 6 mm, 5 μm),with methanol-water( 48∶ 52) as the mobile phase,using the detection wavelength of 440 nm and the flow rate of 1. 0 mL·min-1. Results The calibration curves of crocin-Ⅰ and crocin-Ⅱ were linear in the ranges of 6-45 μg·mL-1( r = 1. 000 0) and 3. 2-24 μg·mL-1( r =1. 000 0),respectively. The average recoveries were 98. 73%( RSD =1. 24%,n =6) for crocin-Ⅰ and 98. 01 %( RSD =1. 53%,n =6) for crocin-Ⅱ. Conclusion The method is simple,accurate and reproducible,which is suitible for the quality control of chongcao baoyuan capsules.

Key concepts: Crocin, Chromatography, Chemistry, High-performance liquid chromatography, Linear relationship, Content determination, Calibration curve, Detection limit

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