2013•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Crocin-I and Crocin-II in Honghuayiqiyangxue Capsules

MA Hui-pin

Abstract

Objective To establish an HPLC method for the content determination of crocin-Ⅰand crocin-Ⅱin Honghuayiqiyangxue capsules. Methods A Hypersil ODS2 C 18 column(150 mm × 4. 6 mm,5 μm) was used with methanol-water(48∶ 52) as the mobile phase. The detection wavelength was 440 nm. The flow rate was 1. 0 ml·min-1. Results The calibration curves of crocin-Ⅰand crocin-Ⅱwere linear in the range of 1245 μg·ml-1(r =1. 0000) and 6. 424 μg·ml1(r =0. 9999),respectively. The average recovery was 99. 44%(RSD =1.19%,n =6) for crocin-Ⅰand 98. 79%(RSD = 1. 38%,n = 6) for crocin-Ⅱ. Conclusion HPLC can determine the content of crocin-Ⅰ and crocin-Ⅱin Honghuayiqiyangxue capsules efficiently and accurately.

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Key concepts: Crocin, Chromatography, High-performance liquid chromatography, Chemistry, Linear relationship, Calibration curve, Detection limit, Mathematics