Effect of rh-endostatin on proliferation and apoptosis of nasopharyngeal carcinoma cell CNE2

Objective To evaluate the inhibitory effect and the possible mechanism of rh-endostatin(rh-ES) on nasopharyngeal carcinoma cells(CNE2).Methods CNE2 cells were treated with rh-ES of different concentrations(25,50,100,200 and 400 μg /ml,respectively) in vitro.Methyl thiazolyl tetrazolium(MTT) assay was used for detecting the cell proliferation in rh-ES treatment at different concentrations and different time intervals.Flow cytometry was used for detecting the effect of rh-ES on cell cycle and apoptosis.Transmission electron microscope was used for observing the cellular morphology of CNE2 cells.Results ① MTT detection showed that rh-ES significantly inhibited the growth of nasopharyngeal carcinoma cell CNE2 in both time-and dose-dependent manners.72 hours after rh-ES treatment it was at 400 μg/ml,and the inhibitory rate was 41.89%,which was higher than those of the other groups.② Flow cytometry showed that the proportion of CNE2 cells arrested in G0/G1 phase and cell apoptosis rate after rh-ES treatment were significantly higher than those of the control group respectively(P0.05).③ Transmission electron microscope identified the characteristics of apoptosis appeared in CNE2 cell of rh-ES group,such as crenation of cell organ,chips of chromatin margination and disappearance of caryotheca.Conclusion By inducing apoptosis and changing the cell cycle distribution,rh-ES can inhibit the proliferation of nasopharyngeal carcinoma cell CNE2 in both time-and dose-dependent manners.