Effect of Jianxi Zhuanggu Formula on Apoptosis of Cartilage Cells and Regulating Gene Expressions of P53 and Bcl-2 in Rats with Knee Osteoarthritis

Objective:To clear the cure effect of Jianxi Zhuanggu formula on rats with knee osteoarthritis and explore the treatment mechanism.To provide experimental basis for the development of new drugs of TCM.Methods:60 rats were divided into normal group A(10 cases)and surgical group(50 cases)randomly.Rat knee osteoarthritis model was induced with the improved method of Hulth.Surgical method and the model rats were randomly divided into control group B,Zhuangguguanjie Pill positive control group C,high dose of Jianxi Zhuanggu group D,moderate dose of Jianxi Zhuanggu group E and low dose of Jianxi Zhuanggu group F.7 days after modeling,rats in high,moderate and low dose of Jianxi Zhuanggu groups were given drugs respectively every day(2mL/100g weight),dosages was equal to 8g,4g,and 2g crude drugs respectively,while rats in positive control group were given Zhuangguguanjie pills(2mL/100g weight),dosage was equal to 2g crude drug.Normal group and the model group took equal amounts of saline solution filling respectively.Half rats were killed 1 and 2 months later.The cartilage of lateral femoral condyle was taken to measure apoptosis of cartilage cells and regulation of expressions of P53 and Bcl-2.Results:Experiment group demonstrated the ability of Jianxi Zhuanggu formula and Zhuangguguanjie pills converted the apoptosis trend of the cartilage cells compared to other groups.It was superior to model group in down regulating the hyperexpression of gene P53 and turning up the suppressed Bcl-2 expression.There was a significant difference(P0.05).Conclusions:Cartilage apoptosis is one of the important pathogenesis of osteoarthritis.Janxi Zhuanggu formula can convert the apoptosis trend of the cartilage cells.This may be related to the influence of gene expressions of P53 and Bcl-2 which protect cartilage cells to some extent and prevent development of osteoarthritis.