Influence of Haitongpi Decoction on Apoptosis of Cartilage Cells in Experimental Rabbit Knee Osteoarthritis

Objective:To investigate the effect of Chinese compound prescription Haitongpi Decoction on apoptosis of cartilage cells in experimental rabbit knee osteoarthritis.Methods:Among forty -five New Zealand rabbits,ten were chosen randomly as control group.Another thirty-five were established knee osteoarthritis model using modified Hulth method. One week later,thirty without infection rabbits were chosen and randomly divided into 3 groups:osteoarthritis model group,vapor treated group,and Haitongpi Decoction treated group.The apoptosis of cartilage cells was detected by Flow cytometry 4 weeks later.Results:The apoptosis rate of cartilage cells was 18.57%in control group,29.41%in osteoarthritis model group,28.26%in vapor treated group,and 21.09%in Haitongpi Decoction treated group.Compared with control group,the apoptosis rates of cartilage cells in osteoarthritis model group,vapor treated group and Haitongpi Decoction treated group had significant difference(P0.05).Compared with osteoarthritis model group,the apoptosis rates of cartilage cells in vapor treated group and Haitongpi Decoction treated group had significant difference(P0.05 ).Compared with vapor treated group,the apoptosis rate of cartilage cells in Haitongpi Decoction treated group had significant difference(P0.05).Conclusion:It was considered that Haitongpi Decoction could significantly reduce the apoptosis rate of cartilage cells,potentially delay the degeneration of joint cartilage and accelerate the cartilage repair.