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2008•Journal of Zhaoqing UniversityRequires access

Determination of Vanillin in Food by Ultraviolet Spectrophotometry

Zhao Jian-fen

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Abstract

A simple method for the detection of vanillin in food by UV spectrophotometry was developed.Effects of pH,organic solvents,buffer type and concentration,surfactant type and concentration have been investigated.The optimum conditions of detection for vanillin was 0.5 mol/L NaHCO3-Na2CO3(pH 8.0) buffer with ethanol as a solvent.The wavelength for detection was set at 343.65 nm.Linear equation was y= 0.116 7x+0.254 2(R=0.999 2),linear range was covered from 0.40 mg/L to 12.00 mg/L.The average recovery of method was more than 97.0%,while RSD was less than 2%.The results were accurate and reliable.

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What this paper is about

A simple method for the detection of vanillin in food by UV spectrophotometry was developed.Effects of pH,organic solvents,buffer type and concentration,surfactant type and concentration have been investigated.The optimum conditions of detection for vanillin was 0.5 mol/L NaHCO3-Na2CO3(pH 8.0) buffer with ethanol as a solvent.The wavelength for detection was set at 343.65 nm.Linear equation was y= 0.116 7x+0.254 2(R=0.999 2),linear range was covered from 0.40 mg/L to 12.00 mg/L.The average recovery of method was more than 97.0%,while RSD was less than 2%.The results were accurate and reliable.

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Available abstract

A simple method for the detection of vanillin in food by UV spectrophotometry was developed.Effects of pH,organic solvents,buffer type and concentration,surfactant type and concentration have been investigated.The optimum conditions of detection for vanillin was 0.5 mol/L NaHCO3-Na2CO3(pH 8.0) buffer with ethanol as a solvent.The wavelength for detection was set at 343.65 nm.Linear equation was y= 0.116 7x+0.254 2(R=0.999 2),linear range was covered from 0.40 mg/L to 12.00 mg/L.The average recovery of method was more than 97.0%,while RSD was less than 2%.The results were accurate and reliable.

Key concepts: Vanillin, Spectrophotometry, Chemistry, Chromatography, Solvent, Linear range, Pulmonary surfactant, Detection limit

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