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2006•Chinese Journal of MisdiagnosticsRequires access

Influence of CSA Upon Maturation and Allostimulatory Activity of Murine Bone Marrow-Derived Dendritic Cells in Vitro

Linlin Ma

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Abstract

Objective:To explore the influence of CSA upon the maturation and allostimulatory activity of murine bone marrow-derived dendritic cells and the underlying mechanisms.Methods:Cultured DC were treated with CSA at doses of 0.5 and 1.0 μg/ml.Surface phenotype was assessed by flow cytometry.Immunostimulatory was measured by mixed lymphocyte reaction.Results:DCs cultured in the presence of MPA expressed low levels of CD40 and CD80 and exhibited weak activity in stimulating the proliferation of allogeneic T cells.Conclusion:CSA interfere with immune responses at the earliest stages by impacting dendritic-cell activation,dendritic cells are emerging pharmacological targets of CSA.【

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What this paper is about

Objective:To explore the influence of CSA upon the maturation and allostimulatory activity of murine bone marrow-derived dendritic cells and the underlying mechanisms.Methods:Cultured DC were treated with CSA at doses of 0.5 and 1.0 μg/ml.Surface phenotype was assessed by flow cytometry.Immunostimulatory was measured by mixed lymphocyte reaction.Results:DCs cultured in the presence of MPA expressed low levels of CD40 and CD80 and exhibited weak activity in stimulating the proliferation of allogeneic T cells.Conclusion:CSA interfere with immune responses at the earliest stages by impacting dendritic-cell activation,dendritic cells are emerging pharmacological targets of CSA.【

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Available abstract

Objective:To explore the influence of CSA upon the maturation and allostimulatory activity of murine bone marrow-derived dendritic cells and the underlying mechanisms.Methods:Cultured DC were treated with CSA at doses of 0.5 and 1.0 μg/ml.Surface phenotype was assessed by flow cytometry.Immunostimulatory was measured by mixed lymphocyte reaction.Results:DCs cultured in the presence of MPA expressed low levels of CD40 and CD80 and exhibited weak activity in stimulating the proliferation of allogeneic T cells.Conclusion:CSA interfere with immune responses at the earliest stages by impacting dendritic-cell activation,dendritic cells are emerging pharmacological targets of CSA.【

Key concepts: CD80, Mixed lymphocyte reaction, CD40, Dendritic cell, Flow cytometry, Bone marrow, In vitro, Cell biology

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