Anti-viral Effects of Qingdupian Granule on Influenza Virus H1N1 in Vitro

In order to investigate the inhibitory action of Qingdupian granule on influenza A virus H1N1 under the different drug delivery modes,MDCK(Madin-Darby canine kidney)cells were taken as influenza A virus H1N1 host.Under three different drug delivery modes,virus infection prior to drugs,drugs action before virus infection,adding drug and virus at the same time,3repeats of each mode,the inhibitory effect of Qingdupian granule on influenza A virus H1N1 was determined through the cytopathic effect(CPE)and MTT colorimetric method(MTT).And Amantadine was positive drug control group of the experiment.From the results,Qingdupian granule showed different ways of effect on anti-influenza A virus H1N1,such as inhibiting the biosynthesis of influenza virus H1N1,the absorption to influenza virus,the blocking of the accession to cells and killing influenza virus directly.The median effective concentration was 411μg/mL,434μg/mLand 532μg/mL(TI=3.5)of inhibiting the biosynthesis of avian influenza virus H1N1 respectively of 3repeats;The median effective concentration was 404μg/mL,458μg/mL,467μg/mL(TI=3.6)of the absorption to influenza virus,the blocking of the accession to cells of 3repeats;The median effective concentration was 479μg/mL,481μg/mL and 461μg/mL(TI=3.4)of killing influenza virus directly of 3repeats.Qingdupian granule has a significant inhibitory effect on influenza A virus H1N1,it will provide experimental basis for treatment and clinical administration.