Determination of Paeonol in Cortex moutan by RP-HPLC

OBJECTIVE To establish a new internal standard RP-HPLC method with cinnamic acid as internal standard for quantitation of paeonol in Cortex moutan.METHODS The determination by RP-HPLC was carried out using ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm) at room temperature,with mobile phase of methanol-water-acetic acid(50∶50∶0.5) at a flow rate of 1 mL·min-1.The detection wavelength was set at 280 nm.RESULTS Excellent linear correlation was established with the range of 5.85～117 mg·L-1(r=0.999 9).The average recovery of this method was 101.0%,and RSD was 1.5%.CONCLUSION The method can be applied in quantitive determination of Cortex moutan or preparations contained Cortex moutan with good accuracy and reliability.