ScholarLens Back to search
2014•Strait Pharmaceutical JournalRequires access

Determination of paeonol in extract of Cortex Moutan by RP-HPLC

Jinglian Liu

Open publisher page 0 citations

Abstract

OBJECTIVE To develop a RP-HPLC method for the determination and analysis of paeonol in extract of cortex moutan. METHODS The content of Paeonol was determined by RP-HPLC method. The analysis was performed on a RP-HPLC apparatus equipped with a Inertsil ODS-3 C 18 column( 4. 6mm × 250mm,5μm),The DAD detection was carried out at the wavelength of 274nm and a temperature of 30℃,the mobile phase consisted of methanol-water( 60∶ 40) with the flow rate of 0. 8mL·min- 1. RESULTS The calibration curve of paeonol was linear in the ranges of 35. 8μg·mL- 1( R2= 0. 9999). The average recovery of paeonol was 98. 54%( RSD = 1. 03%,n = 6). The sample was extracted with water-steam distillation after ultrasonication 30min. CONCLUSION The method is effective,accurate,and can be applied to quantitatively analyse paeonol in extract of cortex moutan.

About this research paper

What this paper is about

OBJECTIVE To develop a RP-HPLC method for the determination and analysis of paeonol in extract of cortex moutan. METHODS The content of Paeonol was determined by RP-HPLC method. The analysis was performed on a RP-HPLC apparatus equipped with a Inertsil ODS-3 C 18 column( 4. 6mm × 250mm,5μm),The DAD detection was carried out at the wavelength of 274nm and a temperature of 30℃,the mobile phase consisted of methanol-water( 60∶ 40) with the flow rate of 0. 8mL·min- 1. RESULTS The calibration curve of paeonol was linear in the ranges of 35. 8μg·mL- 1( R2= 0. 9999). The average recovery of paeonol was 98. 54%( RSD = 1. 03%,n = 6). The sample was extracted with water-steam distillation after ultrasonication 30min. CONCLUSION The method is effective,accurate,and can be applied to quantitatively analyse paeonol in extract of cortex moutan.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To develop a RP-HPLC method for the determination and analysis of paeonol in extract of cortex moutan. METHODS The content of Paeonol was determined by RP-HPLC method. The analysis was performed on a RP-HPLC apparatus equipped with a Inertsil ODS-3 C 18 column( 4. 6mm × 250mm,5μm),The DAD detection was carried out at the wavelength of 274nm and a temperature of 30℃,the mobile phase consisted of methanol-water( 60∶ 40) with the flow rate of 0. 8mL·min- 1. RESULTS The calibration curve of paeonol was linear in the ranges of 35. 8μg·mL- 1( R2= 0. 9999). The average recovery of paeonol was 98. 54%( RSD = 1. 03%,n = 6). The sample was extracted with water-steam distillation after ultrasonication 30min. CONCLUSION The method is effective,accurate,and can be applied to quantitatively analyse paeonol in extract of cortex moutan.

Key concepts: Paeonol, Chromatography, Chemistry, High-performance liquid chromatography, Calibration curve, Steam distillation, Distillation, Detection limit

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of paeonol in extract of Cortex Moutan by RP-HPLC — Research Paper | ScholarLens