2008•Cereals & OilsRequires access

Determination of ursolic acid and oleanolic acid in seeds of perilla frutescens(L. )britton by RP-HPLC

Chen Wu

Abstract

The contents of ursolic acid and oleanolic acid in seeds of Perilla frutescens (L.) Britton were determined by RP-HPLC. Ursolic acid and oleanolic acid was separated and analyzed on Kromasil C18 column (250 mm×4.6 mm, 5μm) using methanol-water (87:13)as mobile phase at flow rate of 0.8 mL/min. UV detection was at 210 nm and the column temperature was 28℃. An average recovery of 100.6% was obtained with a RSD of 1.6% in the range of 0.10～4.00μg for ursolic acid, and an average recovery or 98.7% was obtained with a RSD of 1.2% in the range of 0.05～2.00μg for oleanolic acid. The method is sensitive, fast and accurate. It can be used for the determination of ursolic acid and oleanolic acid.

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Available abstract

Key concepts: Ursolic acid, Oleanolic acid, Perilla frutescens, Chromatography, Chemistry, High-performance liquid chromatography, Methanol, Biochemistry