Effects of apoptosis and Bcl-2 expression by celecoxib with Cisplatin in SGC-7901 human gastric cancer cell line

Objective:To study the effects of celecoxib,a selective COX-2 inhibitor,with cisplatin on cell proliferation,Bcl-2 Expression and apoptosis in SGC-7901 human gastric cancer cell line and to explore its anti-neoplasm mechanism.Methods:MTT assay was used to determine cell proliferation after incubation in different concentrations(100,200,300μmol/L) of celecoxib with cisplatin(2.0 μg/mL).Flow cytometry was adopted to examine apoptotic rate,cell cycle and Bcl-2 level.Results:Celecoxib significantly inhibited the growth of SGC-7901 human gastric cancer cells and elevated the inhibiting rate compared with control group(P0.05).The apoptotic peak was detected with FCM and cells were blocked in G0/G1 stage.The inhibiting effects were in dose and time dependent manner,and were associated with decrease of Bcl-2 level.Conclusion:COX-2 inhibitor celecoxib can accelerate the apoptosis induced by cisplatin and inhibite the proliferation in SGC-7901 human gastric cancer cell line.The results indicate one of the mechanisms of the anti-cancer effect of COX-2 inhibitor.