Effects of Apoptosis and Bcl-2 and Bax Expression by Celecoxib with Cisplatin in SGC-7901 Human Gastric Cancer Cell Line

Objective To investigate the effects of Celecoxib,a selective COX-2 inhibitor,with 5-Fu on cell proliferation,Bcl-2 and Bax Expression and apoptos is in SGC-7901 human gastric cancer cell line and to explore its anti-neoplasm mechanism.Methods MTT assay was used to determine cell proliferation after incubation in different concentrations of celecoxib with 5-Fu.The effect of celecoxib with 5-Fu on cell cycle changes and apoptosis of cells was studied by flow cytometry(FCM).The immunohistochemistry measured the expression of Bcl-2 and Bax proteins.Results Celecoxibs with 5-Fu significantly inhibited the growth of gastric cancer cells and elevated the inhibiting rate compared with control group(P0.05).Flow cytometry apoptotic peak was detected,the inhibition was time-dose dependent;Treated gastric cancer cells were detected by immunohistochemistry and found that the combination group had more significant decreased expression of Bcl-2 and Bax expression increased.Conclusion Celecoxib combined with 5-Fu had synergistic inhibitory effect on gastric cancer cells,which may change the Bcl-2 and Bax expression and the level.