Study on the Effects of Apoptosis and Telomerase Activity by Celecoxib in SGC-7901 Human Gastric Cancer Cell Line

Objective To study the effects of celecoxib,a selective COX-2 inhibitor,on cell proliferation,telomerase activity and apoptosis in SGC-7901 human gastric cancer cell line and to explore its anti-neoplasm mechanism.Methods MTT assay was used to determine cell proliferation after incubation in different concentrations(100,200,300 μmol/L) of celecoxib.Telomerase activity was detected by semi-TRAP assay.flow cytometry was adopted to examine apoptotic rate,cell cycle and bcl-2 level.Results Celecoxib inhibited significantly the growth of SGC-7901 human gastric cancer cells and elevated the inhibiting rate(P0.05) as compared with control groups.The apoptotic peak was detected with FCM and cells were blocked in G_0/G_1 stage.The telomerase activity was significantly inhibited in dose and time dependent manner,and the inhibition of telomerase activity was associated with decrease of bcl-2 level.Conclusion COX-2 inhibitor celecoxib not only inhibited proliferation,but induced apoptosis and inhibited telomerase activity by partly decreasing bcl-2 level in SGC-7901 human gastric cancer cell line.The results indicate one of the mechanisms underlying the anti-cancer effect of COX-2 inhibitor.