Celecoxib induces apoptosis and autophagy of gastric cancer SGC-7901 cells

Objective:To observe the effect of celecoxib on apoptosis and autophagy of human gastric cancer cell line SGC-7901,and to investigate the mechanism of apoptosis.Methods: SGC-7901 cells were treated with different concentrations of celecoxib,proliferation of SGC-7901 cells was studied by MTT assay,apoptosis was assessed by TUNEL,ultrastructure changes was observed by transmission electron microscopy,apoptotic rate was examined by flow cytometry,and expression of caspase-8,caspase-9 mRNA was analyzed by real-time quantitative PCR.Results: Celecoxib inhibited proliferation of SGC-7901 cells in a time-and dose-dependent manner,with inhibitory rate of(85.6±4.51)% at 125 μmol/L celecoxib for 72 h.Celecoxib induced apoptosis of SGC-7901 cells,typical apoptotic body and autophagosome were observed under TEM,and apoptotic rate increased from(2.2±1.32)% to(35.7±5.73)%(P0.05) as detected by FCM.Expression of caspase-8 and caspase-9 mRNA increased sharply in SGC-7901 cells treated with celecoxib in a time-and dose-dependent manner.Conclusion: Celecoxib can induce apoptosis of gastric cancer SGC-7901 cells by activating caspase-8 in the death-receptor pathway and caspase-9 in the mitochondrial pathway,and induce autophagic cell death.