Effect of Shenshuai Xiezhuo Tang on BMP7/TGFβ1/Smads Signaling Transduction in Renal Interstitial Fibrosis Rats

Objective:Observed the effect of shen Shuai Xie Zhuo tang To the unilateral ureteral obstruction(UUO)big mouse kidney organization on renal expression of bone morphogenetic protein-7(BMP7)、transforming growth factor beta 1(TGF beta 1)/ smads Signaling Pathway.Methods:Divides into stochastically 72 big mice:Sham-operation group,model group,Shen Shuai Xie Zhuo tang low dose team,Shen Shuai XieZhuo tang middle dose team,Shen Shuai Xie Zhuo tang high dose team,benazepril group,uses the unilateral ureteral obstruction(UUO)animal model,after the technique,7th,14th days separately observe the obstruction side big mouse kidney organization pathology change and BMP7、Smad2、Smad6、TGF beta 1、Ⅰ、Ⅲ collagen type and FN protein expression.Results:The expression of Smad2、TGF beta 1、Ⅰ、Ⅲ collagen type and FN of each treatment group big mouse kidney organization was lower than the model group rats,but higher than the sham-operation group rats;But each treatment group big mouse kidney organizes the BMP7、Smad6 protein expression to be opposite,is most obvious effect by the Shen Shuai Xie Zhuo tang high dose team.There was significant negative correlation between BMP7、 smad6 and TGF beta 1;There was significant positive correlation between smad2 and TGF beta 1.Conclusion:The probable mechanism of the therapeutic effect of Shen Shuai Xie Zhuo tang as follows:Enhances the BMP7、 Smad6 protein to express,through suppresses the Smad2 protein expression,adjust the BMP7、TGF beta 1/Smads Signaling Transduction,suppresses TGF beta 1 the production,suppressesⅠ,Ⅲ collagen type and FN and so on ECM excessive depositions,reduce the renal interstitial fibrosis.The treatment is superior to benazepril.