Influence of Yiqi Huoxue Jiangzhuo Decoction on TGF-β_1-smad-ILK Signal Transduction Pathway in Rats with Unilateral Ureteral Obstruction

Objective:To study Yiqi Huoxue Jiangzhuo decoction(YHJD) on transforming growth factor-beta 1(TGF-β1),smad7,p-smad2/3,integrin-linked kinase(ILK) protein expressions,and investigate the effect of YHJD on TGF-β1-smad-ILK signal transduction pathway.Method:Unilateral ureteral obstruction(UUO) method was adopted to establish rat model of renal interstitial fibrosis,one hundred and thirty-two SD rats were randomly divided into normal group,sham operation group,model group,western medicine group,YHJD large,medium and low-dose group.Coomassie brilliant blue method was used to measure 24 h urinary protein.The protein expression of TGF-β1,smad7,p-smad2/3,ILK in kidney tissue were detected respectively by immunohistochemistry.Result:Compared with the normal group and sham operation group,TGF-β1,p-smad2,3,and ILK protein expressions in kidney tissue in he model group were significantly increased at 1,2,3 weeks(P0.01),there were weak expressions of TGF-β1,p-smad2/3,and ILK protein in normal group.TGF-β1,p-smad2/3,and ILK protein expressions in YHJD large,medium,low-dose groups were increased compared with those in the normal group and sham operation group at each time point.However,compared with the model group they were significantly reduced(P0.01),fosinopril sodium group was also significantly reduced than in model group(P0.01).Conclusion:YHJD could block TGF-β1-smad-ILK signal transduction by reducing TGF-β1,p-smad2/3,ILK protein expressions and increasing smad7 proteins expression in kidney tissue.It might be the mechanisms of YHJD relieving renal interstitial fibroblast.